Maximizing linear amplification of nucleic acids with optimized T7 promoters

ABSTRACT: Linear amplification of RNA by T7 bacteriophage polymerase is widely used in molecular biology. We performed 5’RACE-Seq to identify T7 promoter variants with enhanced transcriptional activity that generate up to five-fold higher RNA output in large scale synthesis reactions. In single-cell RNA-Sequencing, optimized T7 promoters facilitate library preparation, and substantially increase library complexity and the number of expressed genes detected per cell, highlighting a particular value for bioanalytical applications

INSTRUMENT(S): Illumina MiniSeq

ORGANISM(S): Homo sapiens

SUBMITTER: Ramon Oliveira Vidal 

PROVIDER: E-MTAB-8508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress