Affymetrix GeneChip™ Canine Gene 1.1ST Array of canine heart valves in all stages of Myxomatous mitral valve disease (Normal-4)

ABSTRACT: Whole mitral valves from each Whitney grade of disease; normal (n=6), grade 1 (n=5), grade 2 (n=6), grade 3 (n=6) and grade 4 (n=5). mRNA was extracted using the qiagen RNeasy minikit and assessed by the agilent 2200 bioanalsyser. all samples passed quality control and transcriptomic analysis was performed by edinburgh genomics using the Affymetrix GeneChip™ Canine Gene 1.1ST Array. Results were reported as CEL files and then analysed in Affymetrix expression console (version 1.4.1.46) using the canine 1.1ST library files from Affymetrix. Robust Multi-array Average (RMA) was used to perform gene level normalisation and signal level summarisation.Following this, quality control assessment was performed on the data set. This included assessment of hybridisation (spike in) controls, labell

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Greg Markby 

PROVIDER: E-MTAB-8618 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress