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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell RNA-seq of intestinal crypts of Red2Kras and Red2Pi3k mice

ABSTRACT: We isolated mutant and wild-type epithelial cells as well as mesenchymal and immune cells from Confetti, R2Kras and R2Pi3k lines at 2 weeks post-induction and generated single-cell RNA-seq data from them.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Benjamin Simons

PROVIDER: E-MTAB-8656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tracing oncogene-driven remodelling of the intestinal stem cell niche.

Yum Min Kyu MK Han Seungmin S Fink Juergen J Wu Szu-Hsien Sam SS Dabrowska Catherine C Trendafilova Teodora T Mustata Roxana R Chatzeli Lemonia L Azzarelli Roberta R Pshenichnaya Irina I Lee Eunmin E England Frances F Kim Jong Kyoung JK Stange Daniel E DE Philpott Anna A Lee Joo-Hyeon JH Koo Bon-Kyoung BK Simons Benjamin D BD

Nature 20210602 7863

Interactions between tumour cells and the surrounding microenvironment contribute to tumour progression, metastasis and recurrence<sup>1-3</sup>. Although mosaic analyses in Drosophila have advanced our understanding of such interactions<sup>4,5</sup>, it has been difficult to engineer parallel approaches in vertebrates. Here we present an oncogene-associated, multicolour reporter mouse model-the Red2Onco system-that allows differential tracing of mutant and wild-type cells in the same tissue. B ...[more]

PMID: 34079126

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