Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

SPGEV

ABSTRACT: Comparison of genomic expression profiles in the medulla oblongata of 4 preclinical naturally scrapie infected sheep and 6 controls.. The experiment consisted of the comparison of gene expression profiles in the medulla oblongata of preclinical scrapie and control sheep using oligoDNA CVI-Agilent custom 4x44K chips hybridizations.

ORGANISM(S): Ovis aries

SUBMITTER: Hicham Filali

PROVIDER: E-MTAB-866 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression profiling and association with prion-related lesions in the medulla oblongata of symptomatic natural scrapie animals.

Filali Hicham H Martin-Burriel Inmaculada I Harders Frank F Varona Luis L Lyahyai Jaber J Zaragoza Pilar P Pumarola Martí M Badiola Juan J JJ Bossers Alex A Bolea Rosa R

PloS one 20110524 5

The pathogenesis of natural scrapie and other prion diseases remains unclear. Examining transcriptome variations in infected versus control animals may highlight new genes potentially involved in some of the molecular mechanisms of prion-induced pathology. The aim of this work was to identify disease-associated alterations in the gene expression profiles of the caudal medulla oblongata (MO) in sheep presenting the symptomatic phase of natural scrapie. The gene expression patterns in the MO from ...[more]

PMID: 21629698

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Project description:The Bashbay sheep (Ovis aries), an indigenous breed of Xinjiang, China, has many excellent characteristics. However, hybrids of Argali sheep and Bashbay sheep are susceptible to Mycoplasma ovipneumoniae infection, the causative agent of mycoplasma ovipneumonia, a chronic respiratory disease that is harmful to the sheep industry. To date, knowledge regarding the mechanisms responsible for M. ovipneumoniae pathogenesis is in scant. Herein, we report the results of transcriptome profiling of lung tissues from Argali hybrid sheep experimentally infected with an M. ovipneumoniae strain at 4 and 14 days post-infection, in comparison to mock-infected animals (0 d). Transcriptome profiling was performed by deep RNA sequencing, using the Illumina platform. The analysis of differentially expressed genes was performed to determine concomitant gene-specific temporal patterns of mRNA expression in the lungs after M. ovipneumoniae infection. We found 156 differentially expressed genes (44 up-regulated, 112 down-regulated) when comparing transcriptomic data at 4 and 0 days post-infection, and 367 (35 up-regulated, 332 down-regulated) when comparing 14 versus 0 days post-infection. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the differentially expressed genes at 4 and 14 versus 0 days post-infection were enriched in 109 and 150 pathways, respectively, and the Primary immunodeficiency pathway was considered most closely related to MO infection (p < 0.01). Hyper-IgM syndrome was identified based on the B-cell immunodeficiency signaling pathway from differentially expressed genes related M. ovipneumoniae infection. Gene Ontology analysis showed that differentially expressed genes in different groups were enriched for 497 and 928 terms, where those most closely related to M. ovipneumoniae infection are ciliated motor damage (p < 0.01). These results could aid in understanding why the Argali hybrid sheep are susceptible to MO as well as how M. ovipneumoniae infection progresses in the lungs and may provide useful information regarding key regulatory pathways.

Dataset Information

SPGEV

Publications

Gene expression profiling and association with prion-related lesions in the medulla oblongata of symptomatic natural scrapie animals.

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