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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of Pseudomonas aeruginosa PAO1 wild-type and mutant containing a single nucleotide polymorphism in fusA1

ABSTRACT: The gene encoding elongation factor G, fusA1, is frequently mutated in clinical isolates of Pseudomonas aeruginosa from patients with cystic fibrosis. Recent work has shown that fusA1 mutants often display elevated aminoglycoside resistance due to increased expression of the aminoglycoside efflux pump, MexXY. We isolated a spontaneous gentamicin-resistant fusA1 mutant (FusA1-P443L) in which mexXY expression was increased. We compared the transcriptome of this fusA1 mutant (EMC1) with the P. aeruginosa PAO1-derived progenitor strain (EMC0) and complemented mutant strain expressing the wild-type fusA1 gene in trans (EMC1*).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Pseudomonas aeruginosa PAO1

SUBMITTER: Eve Maunders

PROVIDER: E-MTAB-8690 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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