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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of mouse small intestinal epithelium at different development stages (E18.5, P7, P21)

ABSTRACT: To assess the role of genes in development in mouse small intestinal epithelium, we isolated intestinal epithelium tissue from wild type mice at E18.5, P7 and P21. RNA was directly isolated used for RNA seq and were able to investigate gene expression at different development stages

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Menno Oudhoff

PROVIDER: E-MTAB-8713 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium.

Zwiggelaar Rosalie T RT Lindholm Håvard T HT Fosslie Madeleine M Terndrup Pedersen Marianne M Ohta Yuki Y Díez-Sánchez Alberto A Martín-Alonso Mara M Ostrop Jenny J Matano Mami M Parmar Naveen N Kvaløy Emilie E Spanjers Roos R RR Nazmi Kamran K Rye Morten M Drabløs Finn F Arrowsmith Cheryl C Arne Dahl John J Jensen Kim B KB Sato Toshiro T Oudhoff Menno J MJ

Science advances 20200911 37

Intestinal epithelial homeostasis is maintained by adult intestinal stem cells, which, alongside Paneth cells, appear after birth in the neonatal period. We aimed to identify regulators of neonatal intestinal epithelial development by testing a small library of epigenetic modifier inhibitors in Paneth cell-skewed organoid cultures. We found that lysine-specific demethylase 1A (<i>Kdm1a/Lsd1</i>) is absolutely required for Paneth cell differentiation. <i>Lsd1</i>-deficient crypts, devoid of Panet ...[more]

PMID: 32917713

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