Directed differentiation of human embryonic stem cells towards anterior neural ectoderm cells using small molecules

ABSTRACT: Here we demonstrate that hES cells can be reproducibly and efficiently induced to PAX6+ and OCT4- cells with the use of small molecules in animal-component free condition. We are also showing by whole genome microarray and single cell QPCR that induced cells express genes characteristic for the development of anterior neuroectoderm, in particular development of future forebrain. Note: The samples included in this experiment were processed in 3 separate batches and we performed a batch correction step in the analysis as indicated in the transformation protocol.

ORGANISM(S): Homo sapiens

SUBMITTER: Alex Gutteridge 

PROVIDER: E-MTAB-872 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress