Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MiRNA microarray analysis to explore the expression profiles of miRNAs using the same liver tissues of NFD-fed mice and HFD-fed mice


ABSTRACT: The miRNA microarray analysis was performed to explore the expression profiles of miRNAs using the same liver tissues of NFD-fed mice and HFD-fed mice  Summary: An abstract of the experiment and the data analysis.  Project Description: Sample and experiment information.  Array Information: miRCURY™ LNA expression array information.  Data Analysis for miRNAs: 1. Low intensity filtering and data normalization: After low intensity miRNAs filtering, raw signal intensities are normalized in Median method. (miRNAs that intensities>=30 in all samples are chosen for calculating normalization factor) 2. Quality assessment of miRNA data after filtering: Contains box plot, Correlation Matrix and scatter plot for miRNAs after normalization. 3. Differentially expressed miRNAs screening: Contains significant differentially expressed miRNAs that pass Volcano Plot filtering. (Fold Change>=1.5, P-value<=0.05) 4. Heat map and hierarchical clustering: Hierarchical clustering on the significant differentially expressed miRNAs that passed Volcano Plots filtering.  Sample RNA Quality Control: Sample quality control data file from Nanodrop 1000 spectrophotometer and standard denaturing agarose gel electrophoresis.  Methods: A brief introduction for microarray, experiment, and data analysis.  FAQ: Frequently asked question Additional miRNA Array Analysis (charge an extra fee):  Prediction Analysis for Microarrays (PAM analysis)  miRNA Target Gene Prediction and Functional Analysis Additional files provided:  Graphs (*.jpg)  Raw Intensity File(*.xls, raw miRNAs signal intensity)  Layout File (*.gal, the files contain information on the positioning of the capture probes on the array and microRNA annotations for your species of interest)  Raw data files produced by GenePix Pro 6.0

ORGANISM(S): Mus musculus

SUBMITTER: Fei Xu 

PROVIDER: E-MTAB-8731 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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