Platform Comparison for miRNA

ABSTRACT: The goal of this study was to investigate the generation and normalization of microRNA expression data based on microarray technologies to comparatively assess their quality. Two profiling platforms were compared: the single-channel Affymetrix GeneChip(r) and the Exiqon dual-channel miRCURY LNA(tm), which was processed as a single-channel array. Due to fundamental differences in the platform constitution, the normalization methods developed for gene expression need to be applied very cautiously to microRNA raw data. This motivated the development of a novel normalization method based on controllable assumptions, which uses the intensities of spike-in control probes. The results showed that the novel normalization method reduced the data variability in the most consistent way and confirmed

INSTRUMENT(S): Affymetrix GeneChip Scanner 3000 7G

ORGANISM(S): Mus musculus

SUBMITTER: Sam Ansari 

PROVIDER: E-MTAB-875 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress