RNA-seq of Oligotropha carboxidovorans strain OM5, a promising candidate for the aerobic utilization of synthesis gas, heterotrophically (acetate) and autotrophically (syngas) grown.

ABSTRACT: Oligotropha carboxidovorans strain OM5 is an aerobic carboxidotrophic bacterium and potentially a promising candidate for such processes. We here performed RNA-Seq analysis comparing cells of this organism grown heterotrophically with acetate or autotrophically with CO2, CO and H2 as carbon and energy source and found a variety of chromosomally and of native plasmid-encoded genes to be highly differentially expressed. For RNA extraction and RNA-Seq, respectively, six aliquots of the sample of each culture replicate were pooled to gain enough cell mass and RNA, accordingly, while four biological replicates were processed for the RNA-Seq analysis. Total RNA was isolated from four biological replicates using Zymo Quick-RNA Miniprep Plus kit with bead beating and DNase-treatment (Zymo Research

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Oligotropha carboxidovorans OM5

SUBMITTER: Bernhard J. Eikmanns 

PROVIDER: E-MTAB-8786 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress