ChIP-seq for Leu3 with addition of different levels of leucine, valine and isoleucine

ABSTRACT: In this experiment we wanted to see how the binding behavior of the S. Cerevisiae transcription factor Leu3, on of the main regulators of leucine biosynthesis, is affected by different availability of the branched chain amino acids. For this we grow the cells in shake flask under glucose limitation and treated them 2 hours before sampling. The cells were then cross-linked with formaldehyde and ChIP-seq was performed using the Oxford Nanopore MinIon.

INSTRUMENT(S): MinION

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jens Nielsen 

PROVIDER: E-MTAB-8820 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress