Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Fractionation of different lignocelullose by Streptomyces coelicolor A3(2) and production of secondary metabolites

ABSTRACT: The goal was to study the dfactionation of different lignocelullose (glucose, wheat bran, wheat straw) by Streptomyces coelicolor A3(2) and the corresponding production of secondary metabolites. This was performed by multi-omic experiment such as transcriptomic/metabolomic and leads to the production of new metabolites. For that, the strain Streptomyces coelicolor A3(2) was subjected to two carbon sources in triplicate (wheat bran and glucose as control). Enzymatic activities were studied at different times and the expression of CAZYmes was studied by transcriptomic in order to detect which enzymes are needed for each carbon source

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Ludovic Besaury

PROVIDER: E-MTAB-8971 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Borodina2005 - Genome-scale metabolic network of Streptomyces coelicolor (iIB711) This model is described in the article: Genome-scale analysis of Streptomyces coelicolor A3(2) metabolism. Borodina I, Krabben P, Nielsen J. Genome Res. 2005 Jun; 15(6): 820-829 Abstract: Streptomyces are filamentous soil bacteria that produce more than half of the known microbial antibiotics. We present the first genome-scale metabolic model of a representative of this group--Streptomyces coelicolor A3(2). The metabolism reconstruction was based on annotated genes, physiological and biochemical information. The stoichiometric model includes 819 biochemical conversions and 152 transport reactions, accounting for a total of 971 reactions. Of the reactions in the network, 700 are unique, while the rest are iso-reactions. The network comprises 500 metabolites. A total of 711 open reading frames (ORFs) were included in the model, which corresponds to 13% of the ORFs with assigned function in the S. coelicolor A3(2) genome. In a comparative analysis with the Streptomyces avermitilis genome, we showed that the metabolic genes are highly conserved between these species and therefore the model is suitable for use with other Streptomycetes. Flux balance analysis was applied for studies of the reconstructed metabolic network and to assess its metabolic capabilities for growth and polyketides production. The model predictions of wild-type and mutants' growth on different carbon and nitrogen sources agreed with the experimental data in most cases. We estimated the impact of each reaction knockout on the growth of the in silico strain on 62 carbon sources and two nitrogen sources, thereby identifying the "core" of the essential reactions. We also illustrated how reconstruction of a metabolic network at the genome level can be used to fill gaps in genome annotation. This model is hosted on BioModels Database and identified by: MODEL1507180049. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

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Fractionation of different lignocelullose by Streptomyces coelicolor A3(2) and production of secondary metabolites

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