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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNAseq of naive CD4 T cells deficient for Elp3 activated for 18h against WT control

ABSTRACT: CD4 T cell deficient for the Elp3 gene have a delayed cell cycle entry. We explored by RNAseq by which molecular pathway the absence of Elp3 delayed the cell cycle entry. Naive T cell from CD4-cre Elp3 fl/fl mice and WT littermate were purified and activated in vitro with CD3 and CD28 antibodies for 18h.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Pierre Lemaitre

PROVIDER: E-MTAB-9057 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNAseq of follicular helper T cell from Elp3 deficient mice after Complete Freund Adjuvant immunisation

Project description:Loss of Elp3 in CD4 T cells hindered the development of an optimal T follicular helper T cell (TFH) response. To understand the molecular pathway by which Elp3 is necessary for TFH responses, mice deficient for Elp3 in T cells and WT littermate were immunized with ovalbumin emulsified with complete Freund Adjuvant. 8 days later, TFH were sorted from the draining lymph nodes and submitted for RNAseq

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