RBP9-TAP RIPseq in Trypanosoma brucei

ABSTRACT: RBP9-TAP was expressed in bloodstream T. brucei and bound mRNA was pulled down using IgG beads and eluted via TEV-protease cleavage. rRNA was depleted using RNAseH and rRNA hybridizing oligos.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Christine Clayton 

PROVIDER: E-MTAB-9093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress