DNA replication profiling by BrdU Immunoprecipitation of S.cerevisiae wild-type versus H3K37R mutant

ABSTRACT: Wild-type and isogenic H3K37R yeast cultures were sinchronized in G1 with alpha factor. Cell were released into the cycle in the presence of 200mM Hydroxyurea and 0.5mg/ml BrdU. After 1h 15 minutes at 30C, replication was stopped by addition of NaN3 and cells were processed for DNA immunoprecipitation with anti BrdU antibody.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Namshik Han 

PROVIDER: E-MTAB-9097 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress