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Response of several cell lines originated from liver, skin and colon to stimulation by IFNg, hyper-IL6, IL27 and OSM, investigated at microRNA level

ABSTRACT: In this study 6 cell lines originated from liver, skin and colon tumours as well as 3 non-tumour cell lines from the same organs were profiled with and without stimulation by 4 cytokines: IFNg, hyper-IL6, IL27 and OSM. The main objective was investigation of downstream effects of the stimulation if these cancer cell lines as well as in healthy tissues at microRNA level.

ORGANISM(S): Homo sapiens

SUBMITTER: Iris Behrmann

PROVIDER: E-MTAB-9118 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Response of several cell lines originated from liver, skin and colon to stimulation by IFNg, hyper-IL6, IL27 and OSM, investigated at mRNA level

Project description:In this study 6 cell lines originated from liver, skin and colon tumours as well as 3 non-tumour cell lines from the same organs were profiled with and without stimulation by 4 cytokines: IFNg, hyper-IL6, IL27 and OSM. The main objective was investigation of downstream effects of the stimulation if these cancer cell lines as well as in healthy tissues.

2018-10-01 | E-MTAB-6080 | biostudies-arrayexpress

Crosstalk between very different family members: How STAT3 activating IL6-type cytokines inhibit the IFNg-like responses of IL27 in hepatocellular carcinoma cells

Project description:Interleukin-27 (IL27) is a type-I-cytokine of the IL6/IL12 family predominantly secreted by activated macrophages and dendritic cells. Best understood are the effects of IL27 on immune cells where it has been described to have pro- or anti-inflammatory properties, either promoting TH1 responses or suppressing TH1 and TH2 responses. The action on other cell types is less well studied. In the liver, IL27 expression was observed to be upregulated in patients with hepatitis B, and sera of hepatocellular carcinoma (HCC) patients contain significantly elevated levels of IL27 compared to healthy controls or patients with hepatitis and/or liver cirrhosis. We previously reported interferon-gamma-like antiviral activity of IL27 in hepatic cells. Here we further studied the effects of IL27 on HCC cells in comparison to other cytokines. We were especially interested in the relevance of the IL27-induced STAT3 activation. Thus we compared its response with those induced by IFNg (STAT1-dominated response) or IL6-type cytokines (IL6, Hyper-IL6 or OSM) (STAT3-dominated response). We find that in hepatocytes, IL27 induces an IFNg-like, STAT1-dependent transcriptional response, but we do not find an effective STAT3-dependent response. The availability of STAT1 seems critical in the shaping of the IL27 response, as the siRNA knock-down of STAT1 revealed its ability to induce the acute-phase protein gamma-fibrinogen, a typical IL6 family characteristic. Moreover, we describe a crosstalk between the signaling of IL6-type cytokines and IL27: responses to the gp130-engaging cytokine IL27 (but not those to IFNs) can be inhibited by IL6-type cytokine pre-stimulation, likely by a SOCS3-mediated mechanism. This experiment represents microarray analysis of three hepatocellular carcinoma cell lines treated with IL-27, IFNg, hyper-IL6 or OSM in combination with shRNA knockdown of STAT3.

2017-07-05 | E-MTAB-4570 | biostudies-arrayexpress

Primary healthy liver cells under hyper-IL6 stimulation (miRNA)

Project description:In this study, primary health liver cells obtained from patients with colon cancer and liver metastasis were stimulated with hyper-IL-6 for 6, 24, 48 and 72 h or kept untreated. Both mRNA and miRNA levels were characterized (this is miRNA dataset).

2019-02-28 | E-MTAB-6572 | biostudies-arrayexpress

Intraperitoneal activation of myeloid cells clears ascites and reveals IL27-dependent regression of metastatic ovarian cancer.

Project description:Patients with metastatic ovarian cancer (OvCa) have a 5-year survival rate of less than 30% due to persisting dissemination of chemoresistant cells in the peritoneal fluid and the immunosuppressive microenvironment in the peritoneal cavity. Here, we report that intraperitoneal administration of β-glucan and IFNγ (BI) induced robust tumor regression in clinically relevant models of metastatic OvCa. BI induced tumor regression by controlling fluid tumor burden and activating localized antitumor immunity. β-glucan alone cleared ascites and eliminated fluid tumor cells by inducing intraperitoneal clotting in the fluid and Dectin-1-Syk-dependent NETosis in the omentum. In omentum tumors, BI expanded a novel subset of immunostimulatory IL27+ macrophages and neutralizing IL27 impaired BI efficacy in vivo. Moreover, BI directly induced IL27 secretion in macrophages where single agent treatment did not. Finally, BI extended mouse survival in a chemoresistant model and significantly improved chemotherapy response in a chemo-sensitive model. In summary, we propose a new therapeutic strategy for the treatment of metastatic OvCa.

2024-10-22 | GSE278380 | GEO

Il27

Project description:Rattus norvegicus Il27, interleukin 27 [Source:RGD Symbol;Acc:1561420], is expressed in 3 baseline experiment(s);

| ENSRNOG00000019170 | ExpressionAtlas

IL27

Project description:Sus scrofa IL27, interleukin 27 [Source:VGNC Symbol;Acc:VGNC:89101], is differentially expressed in 2 experiment(s);

| ENSSSCG00000039300 | ExpressionAtlas

IL27

Project description:Macaca mulatta IL27, interleukin 27 [Source:VGNC Symbol;Acc:VGNC:99516], is expressed in 2 baseline experiment(s);

| ENSMMUG00000045351 | ExpressionAtlas

Il27

Project description:Mus musculus Il27, interleukin 27 [Source:MGI Symbol;Acc:MGI:2384409], is differentially expressed in 30 experiment(s);

| ENSMUSG00000044701 | ExpressionAtlas

IL27

Project description:Homo sapiens IL27, interleukin 27 [Source:HGNC Symbol;Acc:HGNC:19157], is expressed in 22 baseline experiment(s);

| ENSG00000197272 | ExpressionAtlas

IL27

Project description:Sus scrofa IL27, interleukin 27 [Source:VGNC Symbol;Acc:VGNC:89101], is expressed in 1 baseline experiment(s);

| ENSSSCG00000039300 | ExpressionAtlas

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