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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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HT-SELEX of recombinant C2H2 zinc finger domains of Sall4

ABSTRACT: To define the sequence preference of SALL4 C2H2 zinc finger domains, we performed SELEX coupled with high-throughput sequencing (HT-SELEX) using the purified SALL4 ZFC1, ZFC2 and ZFC4 domains combined with no protein control experiment.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): artificial sequences

SUBMITTER: Heng-Yang Lee

PROVIDER: E-MTAB-9236 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SALL4 controls cell fate in response to DNA base composition.

Pantier Raphaël R Chhatbar Kashyap K Quante Timo T Skourti-Stathaki Konstantina K Cholewa-Waclaw Justyna J Alston Grace G Alexander-Howden Beatrice B Lee Heng Yang HY Cook Atlanta G AG Spruijt Cornelia G CG Vermeulen Michiel M Selfridge Jim J Bird Adrian A

Molecular cell 20210105 4

Mammalian genomes contain long domains with distinct average compositions of A/T versus G/C base pairs. In a screen for proteins that might interpret base composition by binding to AT-rich motifs, we identified the stem cell factor SALL4, which contains multiple zinc fingers. Mutation of the domain responsible for AT binding drastically reduced SALL4 genome occupancy and prematurely upregulated genes in proportion to their AT content. Inactivation of this single AT-binding zinc-finger cluster mi ...[more]

PMID: 33406384

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