Primed and naïve marmoset pluripotent stem cells as a model for primate development

ABSTRACT: We derived primed pluripotent stem cell cultures (PSCs) from marmoset blastocyst using conventional human PSC culture conditions (KSR/bFGF on feeders). Naïve marmoset PSCs were established by chemical resetting. In addition, we generated forebrain-derived cells from the frontal lobe of a marmoset neonate. Individual cells were manually isolated with a mouth pipette and subjected to single-cell full-lengths transcriptome profiling using a modified version of Smart-Seq2.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Callithrix jacchus

SUBMITTER: Christopher Penfold 

PROVIDER: E-MTAB-9349 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress