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Sequencing and de novo analysis of Oviductus Ranae initial growth transcriptome using Illumina RNA-Seq Technology

ABSTRACT: To obtain an overview of the Oviductus Ranae gene expression profile during initial growing period, a cDNA sample was prepared from Oviductus Ranae and sequenced using the Illumina sequencing platform

ORGANISM(S): Rana chensinensis

SUBMITTER: Yu Zhao

PROVIDER: E-MTAB-936 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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De Novo Transcriptome Sequencing in Oviductus Ranae of Rana chensinensis Using Illumina RNA-Seq Technology

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Angiogenic and Immunologic Proteins Identified by Deep Proteomic Profiling of Human Choroidal and Retinal Vascular Endothelial Cells

Project description:PURPOSE Diseases that involve choroidal or retinal endothelial vascular cells are leading causes of vision loss: age-related macular degeneration, retinal ischemic vasculopathies and non-infectious posterior uveitis. Proteins differentially expressed by these endothelial cell populations are potential drug targets. We used deep proteomics to define the molecular phenotype of human choroidal and retinal endothelial cells at the protein level. METHODS Choroidal and retinal endothelial cells were separately isolated from 5 human eye pairs by selection on CD31. Total protein was extracted and digested, and peptide fractions were analysed by reverse-phase liquid chromatography tandem mass spectrometry. Peptide sequences were assigned to fragment ion spectra and proteins were inferred using public protein databases. Protein abundance was determined by spectral counting. Protein expression in choroidal versus retinal endothelial cells was compared using edgeR package in R, and annotation enrichment analysis was performed. RESULTS Human choroidal or retinal endothelial cells expressed 5042 non-redundant proteins. Setting the false discovery rate at 5%, 498 proteins (14.4%) of 3454 quantifiable proteins with minimum mean spectral counts of 2.5 were differentially expressed between cell populations. Choroidal and retinal endothelial cells were enriched in angiogenic proteins, and retinal endothelial cells were also enriched in immunologic proteins. CONCLUSIONS This work demonstrates the protein heterogeneity of human choroidal and retinal vascular endothelial cells and provides multiple candidates for further study as novel treatments or drug targets for posterior eye diseases.

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De novo assembly in defense against pests in Pinus massoniana

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