Single cell RNA-seq of the Dppa3-KO mouse embryos at 4-cell stage and 8-cell stage

ABSTRACT: We found that the DNA methylation heterogeneity was largely programmed by the initial DNA methylation state in zygote by building a mathematical model. Further, to clarify the causality of transcriptional expression heterogeneity and DNA methylation heterogeneity, we disrupted DNA methylation status during mouse early embryogenesis by knocked out (KO) DNA methylation-related genes Dppa3 in gamete cells. Then we performed single cell RNA-seq on each cell of the KO embryos in 4-cell stage and 8-cell stage.

INSTRUMENT(S): HiSeq X Ten

ORGANISM(S): Mus musculus

SUBMITTER: Chengchen Zhao 

PROVIDER: E-MTAB-9360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress