Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

11 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identifying crossover locations in Arabidopsis thaliana wild type Col-0 x Ct-1 F2 populations using genotyping-by-sequencing

ABSTRACT: Genomic DNA from 305 Col x Ct F2 individuals was extracted by CTAB and used to generate sequencing libraries as previously described (Hennig et al, 2018 G3). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3; Yelina et al, 2015 Genes & Dev).

INSTRUMENT(S): HiSeq X Ten

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Piotr Ziolkowski

PROVIDER: E-MTAB-9369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Identifying crossover locations in Arabidopsis thaliana msh2 Col-0 x Ct-1 F2 populations using genotyping-by-sequencing

Project description:Genomic DNA from 320 msh2 Col x Ct F2 individuals was extracted by CTAB and used to generate sequencing libraries as previously described (Hennig et al, 2018 G3). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3; Yelina et al, 2015 Genes & Dev).

2020-09-02 | E-MTAB-9370 | biostudies-arrayexpress

Identifying crossover locations in Arabidopsis thaliana sni1 Col-0 x Ct-1 F2 populations using genotyping-by-sequencing

Project description:Genomic DNA from 241 sni1-/- Col x Ct F2 individuals was extracted by CTAB and used to generate sequencing libraries as previously described (Hennig et al, 2018 G3). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3; Yelina et al, 2015 Genes & Dev).

2020-12-31 | E-MTAB-9413 | biostudies-arrayexpress

Identifying crossover locations in Arabidopsis thaliana wild type and taf4b Col × Bur F2 populations using genotyping-by-sequencing

Project description:Genomic DNA from 180 Col/Bur F2 individuals, and 186 taf4b/Bur F2 individuals, was extracted by CTAB and used to generate sequencing libraries as previously described (Ziolkowski et al, 2017 Genes & Dev). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev).

2019-12-31 | E-MTAB-8099 | biostudies-arrayexpress

Identifying crossover locations in Arabidopsis thaliana recq4a recq4b and HEI10 recq4a recq4b Col x Ler F2 populations using genotyping by sequencing

Project description:Genomic DNA from 192 recq4a recq4b and 192 HEI10 recq4a recq4b Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Ziolkowski et al, 2017 Genes & Dev). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev).

2017-11-30 | E-MTAB-5949 | biostudies-arrayexpress

Identifying crossover locations in Arabidopsis thaliana wild type Col x CLC, msh2 Col x CLC and msh2 Col x Ler F2 populations using genotyping by sequencing.

Project description:Genomic DNA from 189 wild type Col x CLC, 191 msh2 Col x CLC or 187 msh2 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Serra et al 2018 PNAS). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al., 2015 G3).

2020-09-02 | E-MTAB-8252 | biostudies-arrayexpress

Identifying crossover locations in an Arabidopsis thaliana Col x Ler F2 population using genotyping by sequencing.

Project description:Genomic DNA from 192 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each library. DNA shearing was carried out for 20 minutes at 37C with 0.4U of DNA Shearase (Zymo research). The barcoded adapters used for library construction are listed in Rowan et al. Each set of 96 libraries was sequenced on one lane of an Illumina NextSeq500 instrument (300-cycle Mid Output run). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al; Yelina et al). Rowan BA, Patel V, Weigel D, Schneeberger K. Rapid and Inexpensive Whole-Genome Genotyping-by-Sequencing for Crossover Localization and Fine-Scale Genetic Mapping. G3 (Bethesda). 2015;5: 38598. Yelina NE, Lambing C, Hardcastle TJ, Zhao X, Santos B, Henderson IR. DNA methylation epigenetically silences crossover hot spots and controls chromosomal domains of meiotic recombination in Arabidopsis. Genes Dev. 2015;29: 2183202.

2016-06-11 | E-MTAB-4657 | biostudies-arrayexpress

Identifying crossover locations in an Arabidopsis thaliana HEI10 Col x Ler F2 population using genotyping by sequencing.

Project description:Genomic DNA from 192 HEI10 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each library. DNA shearing was carried out for 20 minutes at 37C with 0.4U of DNA Shearase (Zymo research). The barcoded adapters used for library construction are listed in Rowan et al. Each set of 96 libraries was sequenced on one lane of an Illumina NextSeq500 instrument (300-cycle Mid Output run). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al; Yelina et al). These data should be compared to a set of wild type control libraries available at E-MTAB-4657. Rowan BA, Patel V, Weigel D, Schneeberger K. Rapid and Inexpensive Whole-Genome Genotyping-by-Sequencing for Crossover Localization and Fine-Scale Genetic Mapping. G3 (Bethesda). 2015;5: 38598. Yelina NE, Lambing C, Hardcastle TJ, Zhao X, Santos B, Henderson IR. DNA methylation epigenetically silences crossover hot spots and controls chromosomal domains of meiotic recombination in Arabidopsis. Genes Dev. 2015;29: 2183202.

2016-08-05 | E-MTAB-4967 | biostudies-arrayexpress

Identifying crossover locations in an Arabidopsis thaliana meiMIGS-PPX1-PPX2 Col x Ler F2 population using genotyping by sequencing

Project description:Genomic DNA from 96 meiMIGS-PPX1-PPX2 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each library. DNA shearing was carried out for 20 minutes at 37°C with 0.4U of DNA Shearase (Zymo research). The barcoded adapters used for library construction are listed in Rowan et al. Each set of 96 libraries was sequenced on one lane of an Illumina NextSeq500 instrument (300-cycle Mid Output run). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al; Yelina et al). These data can be compared to a set of wild type control libraries available at E-MTAB-4657. Rowan BA, Patel V, Weigel D, Schneeberger K. Rapid and Inexpensive Whole-Genome Genotyping-by-Sequencing for Crossover Localization and Fine-Scale Genetic Mapping. G3 (Bethesda). 2015;5: 385–98. Yelina NE, Lambing C, Hardcastle TJ, Zhao X, Santos B, Henderson IR. DNA methylation epigenetically silences crossover hot spots and controls chromosomal domains of meiotic recombination in Arabidopsis. Genes Dev. 2015;29: 2183–202.

2020-12-14 | E-MTAB-9621 | biostudies-arrayexpress

Identifying crossover locations in Arabidopsis thaliana CMT3-11 (Col) x CMT3-7 (Ler) F2 and wild type Col x Ler F2 populations using genotyping by sequencing

Project description:Genomic DNA from 384 CMT3-11 (Col) x CMT3-7 (Ler) F2 and 171 wild type Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each library. DNA shearing was carried out for 20 minutes at 37C with 0.4U of DNA Shearase (Zymo research). The barcoded adapters used for library construction are listed in Rowan et al. Each set of 96 libraries was sequenced on one lane of an Illumina NextSeq500 instrument (300-cycle Mid Output run). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al; Yelina et al).

2017-06-05 | E-MTAB-5476 | biostudies-arrayexpress

Identifying crossover locations in Arabidopsis thaliana asy1/+ Col × Ler F2 populations using genotyping-by-sequencing

Project description:Genomic DNA from 191 asy1/+ Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev; Lawrence et al, 2019 Current Biology).

2020-05-18 | E-MTAB-8725 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data