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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA sequencing comparing the transcriptome of a severe congenital neutropenia (SCN) patient who progressed to AML (SCN-AML) with the SCN phase and healthy controls

ABSTRACT: CD34+ hematopoietic stem progenitor cells (HSPCs) from cryo-preserved blood or bone marrow were FACS sorted in TriZol and RNA was isolated according to the manufacturer’s protocol. SMARTer Ultra Low Input RNA kit for sequencing (Clontech, v4 Cat# 634891) was used to generate cDNA. Sequencing libraries were generated using TruSeq Nano DNA Sample Preparation kits (Illumina, Cat# 20015964), according to the low sample protocol and paired-end sequenced on a HiSeq 2500 or Novaseq 6000 (both Illumina).

INSTRUMENT(S): Illumina HiSeq 2500, Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Patricia Olofsen

PROVIDER: E-MTAB-9381 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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