Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

ABSTRACT: To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide DNA methylation and hydroxymethylation patterns as well as the transcription profiles in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed RNA-seq, reduced representation bisulfite sequencing (RRBS) and hydroxym

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Mus musculus musculus

SUBMITTER: Yihang Shen 

PROVIDER: E-MTAB-9421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress