Single cell RNA-seq of hemagglutinin specific B cells after influenza virus infection: VDJ data

ABSTRACT: Understanding dynamics of antigen specific B cell responses and link between BCR and B cell differentiation is crucial for our ability to direct immune responses and to generate memory and plasma cells responses to protective targets. Here we have infected mice with IAV-PR8 and sacrificed them at different days after infection (7-14-28). 2 naive mice were used as controls (spleen and lungs). From individual spleen, lungs and mediastinal lymph nodes we have sorted Hemagglutinin (HA)-specific IgD- B cells and single cell sequenced RNA and BCR. We identify several known and novel B cell subpopulations forming after infection and find organ-specific difference that persist over the course of the response. We found important transcriptional differences between memory cells in lungs and immune o

INSTRUMENT(S): Illumina MiSeq, NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Davide Angeletti 

PROVIDER: E-MTAB-9478 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress