Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Identification of the mRNAs interacting with the Nup59 nucleoporin using RNA-IP and microarrays in budding yeast

ABSTRACT: To specifically explore such mRNA localization events, we immunopurified the entire Nuclear Pore Complex scaffold using Nup59 as a bait and systematically analyzed all co-isolating mRNAs using Agilent microarrays in the budding yeast Saccharomyces cerevisiae. A mock IP was performed as a control experiment, using a yeast strain in which Nup59 was untagged. Two replicates of the Nup59 IP and of the mock IP were performed. The mRNAs with a significant enrichment (p<0.05 in the bayesian test of the LIMMA package) in the mock IP were removed from the further analyses of the Nup59-IP.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Frederic Devaux 

PROVIDER: E-MTAB-9521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis.

Lautier Ophélie O   Penzo Arianna A   Rouvière Jérôme O JO   Chevreux Guillaume G   Collet Louis L   Loïodice Isabelle I   Taddei Angela A   Devaux Frédéric F   Collart Martine A MA   Palancade Benoit B  

Molecular cell 20210409 11

mRNA translation is coupled to multiprotein complex assembly in the cytoplasm or to protein delivery into intracellular compartments. Here, by combining systematic RNA immunoprecipitation and single-molecule RNA imaging in yeast, we have provided a complete depiction of the co-translational events involved in the biogenesis of a large multiprotein assembly, the nuclear pore complex (NPC). We report that binary interactions between NPC subunits can be established during translation, in the cytopl  ...[more]

Similar Datasets

Proteomics The LARP1 homolog Slr1p controls the stability and expression of proto-5’TOP mRNAs in fission yeast
2023-11-05 | PXD043979 | Pride
Transcriptomics Widespread Generation of Alternative UTRs Contributes to Sex-specific RNA Binding by UNR
2011-10-25 | E-GEOD-33178 | biostudies-arrayexpress
Transcriptomics Tma108, a putative M1 aminopeptidase, is involved in ribosome specialization in Saccharomyces cerevisiae
2015-12-24 | E-MTAB-3881 | biostudies-arrayexpress
Proteomics Analysis of HNRNPC Interactome in MDA-MB231 parental and LM2 cells
2023-02-19 | PXD029560 | Pride
Transcriptomics Genome-wide identification of RNA polymerase, RpoD, and RpoS binding sites in Klebsiella pneumoniae MGH 78578
2012-12-31 | E-GEOD-35927 | biostudies-arrayexpress
Transcriptomics RIP-ChIP analyses of N-acetyltransferases binding preferences in yeast
2022-12-31 | E-MTAB-11772 | biostudies-arrayexpress
Proteomics Interactome of the two T. brucei Poly(A) binding protein paralogs PABP1and PABP2
2018-07-16 | PXD008839 | Pride
Transcriptomics Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster
2011-01-05 | E-GEOD-19744 | biostudies-arrayexpress
Proteomics Nuclear pore complexes mediate subtelomeric gene silencing by regulating PCNA levels on chro atin
2023-06-13 | PXD041496 | Pride
Genomics FabR ChIP-chip on Salmonella enterica subsp. enterica serovar Typhimurium SL1344
2014-12-31 | GSE52877 | GEO