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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Specification of HiPS to in vivo placental cell types

ABSTRACT: Here we identify conditions that efficiently drive specification of primed induced pluripotent stem cells to trophectoderm, named Trophoblast Stem Cell (TS). iPS-derived-TS cells share transcriptional, morphological and functional characteristics with human ex vivo cytotrophoblasts including transcriptional activation of human endogenous retroviruses, expression of COVID-19 associated host factors and the ability to generate multinucleated syncytiotrophoblasts with a large fusion index. At high densities in 5% O2, iPS-derived-TS cells form villi-like structures and express extravillous and syncytiotrophoblast proteins HCG-β, HLA-G. To define the molecular changes associated with specification, we compare temporal single cell RNAseq analysis under three separate conditions: 1) BMP4, 2) BMP4 and inhibition of Wnt, 3) Trophoblast Stem cell condition (TS) activation of EGF and Wnt, inhibition of TGFbeta, HDAC and ROCK signaling.

INSTRUMENT(S): Illumina HiSeq 3000

ORGANISM(S): Homo sapiens

SUBMITTER: Jennifer Erwin

PROVIDER: E-MTAB-9526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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