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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome analysis by Illumina microarrays of the effect of overexpressing H2A.J mutants, or WT H2A.J or canonical H2A, on the transcriptome of WI-38hTERT fibroblasts

ABSTRACT: The intent of the experiment was to test the effect of overexpressing H2A.J-V11A, H2AJ-S123E, H2A.J-S123A, and H2A.J-CterH2A mutants, as well as WT-H2A.J and canonical H2A-type1, on the transcriptome of WI-38hTERT fibroblasts. pTRIPz based lentiviral constructs placing these coding sequences under tetON control were used to produce stable WI38hTERT cell lines for each construct. Expression of the constructs in proliferating cells was induced with 100 ng/ml doxycycline for è days. Transcriptomes were also compared with control proliferating WI-38hTERT and WI-38hTERT cells induced into senescence by incubation with 20 µM etoposide for 2 weeks followed by 1 week without etoposide.

INSTRUMENT(S): Illumina iScan

ORGANISM(S): Homo sapiens

SUBMITTER: Carl MANN

PROVIDER: E-MTAB-9706 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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