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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Cellular and extracellular vesicle RNA sequence for E-cadherin-RFP EMT reporter system expressing mouse breast cancer cell line Py2T during EMT/MET

ABSTRACT: Epithelial-mesenchymal transition (EMT) is a process by which epithelial cells lose cell-cell contact and gain cancer malignancy such as invasion, stemness, chemoresistance and metastasis. Reverse precess, mesenchymal-epithelial transition (MET) is also important for colonization. Extracellular vesicles (EVs) secreted from cancer cells are also important for cancer malignancy. To analyze RNAs from cells and EVs during EMT and MET, RNA sequencing was performed using E-cadherin-RFP/Py2T reporter system.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Yutaro Tsubakihara

PROVIDER: E-MTAB-9750 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TGFβ selects for pro-stemness over pro-invasive phenotypes during cancer cell epithelial-mesenchymal transition.

Tsubakihara Yutaro Y Ohata Yae Y Okita Yukari Y Younis Shady S Eriksson Jens J Sellin Mikael E ME Ren Jiang J Ten Dijke Peter P Miyazono Kohei K Hikita Atsuhiko A Imamura Takeshi T Kato Mitsuyasu M Heldin Carl-Henrik CH Moustakas Aristidis A

Molecular oncology 20220410 12

Transforming growth factor β (TGFβ) induces epithelial-mesenchymal transition (EMT), which correlates with stemness and invasiveness. Mesenchymal-epithelial transition (MET) is induced by TGFβ withdrawal and correlates with metastatic colonization. Whether TGFβ promotes stemness and invasiveness simultaneously via EMT remains unclear. We established a breast cancer cell model expressing red fluorescent protein (RFP) under the E-cadherin promoter. In 2D cultures, TGFβ induced EMT, generating RFP< ...[more]

PMID: 35348275

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