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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single Cell RNA Sequencing of human bone marrow LT-HSCs.

ABSTRACT: 10x single cell sequencing of LT-HSCs (CD34+CD38-CD90+CD45RA-CD49f+) from human bone marrow.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Mari Carmen Romero-Mulero

PROVIDER: E-MTAB-9922 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A Comparison of Whole Genome Gene Expression Profiles of 0p-EpSCs and 15p-EpSCs

Project description:To investigate successful maintenance of skin epithelial stem cells (EpSCs) in vitro using Wnt-3a, we prepared CD49f+CD34+ cells, an EpSC-rich population from adult mouse skin and cultured for 10 days in the presence of Wnt-3a. Sorted CD49f+CD34+ cells proliferated markedly in the presence of Wnt-3a. Those cells were then subjected to a second 10-day culture with Wnt-3a and sorted, with the same procedures repeated until fifteen 10-day cultures were performed. A dye-swapped experiment was performed by hybridizing complimentary RNA (cRNA) labeled with either Cyanine (Cy) -3 or Cy-5 onto 4X44K ver.2 Agilent Whole Mouse Genome Oligo Microarray (G4846A).

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