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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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CRISPR enhancer scan to detect critical elements for the regulation of EVI1 in a translocated enhancer

ABSTRACT: To uncover, in an unbiased fashion, which elements of the 18 kb translocated region control EVI1 transcription, we devised a CRISPR/Cas9-based enhancer scanning approach. We considered all possible sgRNA target sites containing a canonical Cas9 PAM site (NGG) on both strands of the minimal 18 kb translocated region. Deep-sequencing libraries were generated by PCR amplification of sgRNA guide strands using primers that tag the product with standard Illumina adapters and a 4 bp sample barcode in a 2 step-PCR protocol.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Ruud Delwel

PROVIDER: E-MTAB-9948 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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