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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of two human and one cynomologous NPC line to assay activity of DNAse1 hypersensitive sites in the proximity of the Trnp1 gene

ABSTRACT: Homo sapiens and Macaca fascicularis neural progenitor cell lines were transduced with a lentiviral MPRA (Massively Parallel Reporter Assay) library. MPRA barcode sequencing and RNA-seq was performed on the extracted RNA. RNA-seq data was used to confirm transcription factor expression.

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Macaca fascicularis

SUBMITTER: Lucas Esteban Wange

PROVIDER: E-MTAB-9951 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MPRA of two human and one cynomologous NPC line to assay activity of DNAse1 hypersensitive sites in the proximity of the Trnp1 gene

Project description:Homo sapiens and Macaca fascicularis neural progenitor cell lines were transduced with a lentiviral MPRA (Massively Parallel Reporter Assay) library. MPRA barcode sequencing and RNA-seq was performed on the extracted RNA. MPRA data was used to compare activity of regulatory sequences across 75 mammalian species with a focus on primates and correlate these activities with the Phenotype of gyrencephaly.

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