Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

JHI Gp 15k life stage

ABSTRACT: The potato cyst nematodes (PCN), Globodera pallida and Globodera rostochiensis, are important parasites of potato. PCN have complex, biotrophic interactions with their hosts that involve gene expression changes in both the nematode and the host plant. The aim of this study is to determine key genes that are differentially expressed in Globodera pallida life cycle stages and during the initiation of the feeding site in susceptible or partially resistant potato genotypes. For this purpose, two microarray experiments were designed: i) a comparison of eggs, infective second stage juveniles (J2) and sedentary parasitic stage J2s (SJ2); ii) a comparison of SJ2 at eight days after inoculation (DAI) in the susceptible cultivar (Desiree) and two partially resistant lines. The data set in this submission came from experiment (I). The results show differential expression of G. pallida genes during the stages studied, including previously characterized effectors. Also, a large number of genes changed their expression between the SJ2 in the susceptible cultivar compared to those infecting partially resistant lines; the number of genes with modified expression were smaller when two partially resistant lines were compared. Additionally, a histopathological study was performed at several time points (7, 14 and 30 DAI) showing the similarities between both partially resistant lines with a delay and degeneration in the formation of the syncytia in comparison to the susceptible cultivar. Females at 30 DAI in partially resistant lines showed a delay in their development in comparison to the susceptible cultivar.

ORGANISM(S): Globodera pallida

SUBMITTER: Pete Hedley

PROVIDER: E-MTAB-999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:For potato crops, host resistance is currently the most effective and sustainable tool to manage potato root and tuber diseases caused by the plasmodiophorid, Spongospora subterranea. Arguably, zoospore root attachment is the most critical phase of the pathogen infection, however, the mechanisms underlying zoospore root attachment remains unknown. This study investigated the potential role of root cell wall surface polysaccharides and proteins in zoospore root attachment in resistant and susceptible potato cultivars. We first compared the effects of enzymatic removal of root cell wall proteins, N-linked glycans or polysaccharides on S. subterranea attachment to root tissue of resistant and susceptible potato cultivars. Subsequently, mass spectrometry analysis of peptides released by trypsin shaving (TS) of root segments identified 1235 proteins, of which 262 were differentially abundant between the resistant and susceptible cultivars. In particular, proteins associated with glutathione metabolism and lignin biosynthesis were more abundant in the resistant cultivar. Comparison with whole-root proteomic analysis of the same resistant and susceptible cultivars led to identification of 226 proteins unique to the TS dataset, of which 188 were significantly different between cultivars. Among these, the pathogen defence-related cell wall protein stem 28 kDa glycoprotein and two major latex proteins were significantly less abundant in the resistant cultivar compared to the susceptible cultivar. A further major latex protein was detected at reduced levels in the resistant cultivar in both TS and whole-root proteomic datasets. In contrast, in the TS-specific dataset, three glutathione S-transferase proteins were more abundant in the resistant cultivar, while the protein glucan endo-1,3-beta-glucosidase was significantly increased in both the TS and whole-root datasets. These results imply a particular role of major latex proteins and glucan endo-1,3-beta-glucosidase in the regulation of host susceptibility to S. subterranea.