Transcription profiling of wild type mouse lung after treatement with Bleomycin hydrogen chloride to examine disease progression in pulmonary fibrosis

ABSTRACT: Pulmonary fibrosis was induced by a single tail vein injection of Bleomycin hydrogen chloride (100 mg/kg body weight; 1/3 LD50; Nippon Kayaku Co. Ltd., Tokyo) to 6- to 8-wk-old mice. Mice (3-5/endpoint) were sacrificed 7, 15 and 23 days post Bleomycin administration and RNA samples were extracted from sagittal sections of the left lung lobes. Pooled RNA samples, after reverse transcription and labeling, were hybridized to cDNA microarrays, manufactured by RIKEN, containing 21168 gene probes.

ORGANISM(S): Mus musculus

SUBMITTER: Vaggelis Harokopos 

PROVIDER: E-MUGN-1 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress