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Transcription profiling of C. elegans with activated let-60 Ras

ABSTRACT: In Caenorhabditis elegans, let-60 Ras controls many cellular processes, such as differentiation of vulval epithelial cells, function of chemosensory neurons, and meiotic progression in the germ line. Although much is known about the let-60 Ras signaling pathway, relatively little is understood about the target genes induced by let-60 Ras signaling that carry out terminal effector functions leading to morphological change. We have used DNA microarrays to identify 708 genes that change expression in response to activated let-60 Ras.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Beatrice Romagnolo

PROVIDER: E-SMDB-1398 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Downstream targets of let-60 Ras in Caenorhabditis elegans.

Romagnolo Béatrice B Jiang Min M Kiraly Moni M Breton Carrie C Begley Rebecca R Wang John J Lund James J Kim Stuart K SK

Developmental biology 20020701 1

In Caenorhabditis elegans, let-60 Ras controls many cellular processes, such as differentiation of vulval epithelial cells, function of chemosensory neurons, and meiotic progression in the germ line. Although much is known about the let-60 Ras signaling pathway, relatively little is understood about the target genes induced by let-60 Ras signaling that carry out terminal effector functions leading to morphological change. We have used DNA microarrays to identify 708 genes that change expression ...[more]

PMID: 12074557

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Downstream targets of let-60 Ras in Caenorhabditis elegans

Project description:In Caenorhabditis elegans, let-60 Ras controls many cellular processes, such as differentiation of vulval epithelial cells, function of chemosensory neurons, and meiotic progression in the germ line. Although much is known about the let-60 Ras signaling pathway, relatively little is understood about the target genes induced by let-60 Ras signaling that carry out terminal effector functions leading to morphological change. We have used DNA microarrays to identify 708 genes that change expression in response to activated let-60 Ras. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed

2005-07-22 | E-GEOD-2974 | biostudies-arrayexpress

Downstream targets of let-60 Ras in Caenorhabditis elegans

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Project description:Downstream targets of let-60 Ras in Caenorhabditis elegans

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Ras Affects Filamentous Growth in Saccharomyces cerevisiae Solely Through Regulation of Protein Kinase A

Project description:To assess the relative contributions of the PKA and MAPK pathways in Ras stimulation of filamentation, we examined the change in transcriptional profile following activation of Ras in strains in which one of these two pathways has been severed. These arrays contain the experiments involving WT strain with glucose addition, Ras2G19V strain with galactose induction, tpk2-w strain as a control, and RasG19V activation in the tpk2-w background. Activation of Ras in the Σ1278b background – by galactose addition to a gal1 PGAL10-RAS2G19V strain - causes a massive restructuring of the transcription pattern of the cell, which closely resembles the changes induced by addition of glucose. To assess the extent to which these Ras-induced changes depended on signaling through PKA, we compared the expression profile following induction of activated Ras in a wild type background to that following induction of activated Ras in the bcy1 tpk1 tpk3 tpk2V218G. Keywords: time course

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