Transcription profiling of yeast treated with calcium, sodium

ABSTRACT: In Saccharomyces cerevisiae, the Ca2+/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca2+ and Na+, and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a comprehensive analysis of calcineurin/Crz1p-dependent gene expression following addition of Ca2+ (200 mM) or Na+ (0.8 M) to yeast. 163 genes exhibited increased expression that was reduced 50% or more by calcineurin inhibition. These calcineurin dependent genes function in signaling pathways, ion/small molecule transport, cell wall maintenance, vesicular transport, and include many open reading frames of heretofore-unknown function. Three distinct gene classes were defined: 28 genes displayed calci

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Hiroyuki Yoshimoto 

PROVIDER: E-SMDB-15 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress