Transcription profiling of Salmonella enterica serovar Typhimurium strains with mutations in three SPI-2 co-expressed tested for their ability to cause disease in mice

ABSTRACT: Salmonella must express and deploy a type III secretion system located in Salmonella pathogenicity island 2 (SPI-2) in order to survive in host phagocytic vacuoles and to cause systemic infection in mouse models of typhoid fever. A genome-wide approach to screening for Salmonella genes that are transcriptionally co-regulated in vitro with SPI-2 genes was used to identify bacterial loci that might function in a mouse model of systemic disease. Strains with mutations in three SPI-2 co-expressed genes were constructed and tested for their ability to cause disease in mice. We found that virK, a homologue of a Shigella virulence determinant, and rcsC, a sensor kinase, are important at late stages of infection. A second Salmonella gene that has VirK homology, somA, is also important for systemic

ORGANISM(S): Salmonella enterica serovar Typhimurium

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-1981 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress