Transcription profiling of S. pneumoniae D39, a serotype 2 strain of pneumococcus, with that of an isogenic deletion mutant, (delta)luxS, time series

ABSTRACT: We were interested in determining if the LuxS signaling system contributed to the regulation of pneumococcal genes. To accomplish this, we compared the in vitro transcriptional profiles over time of S. pneumoniae D39, a serotype 2 strain of pneumococcus, with that of an isogenic deletion mutant, (delta)luxS, using a spotted S. pneumoniae- specific DNA microarray. For in vitro time course experiments, broth cultures of D39 and (delta)luxS were grown to low density (optical densities between ~0.01- 0.02), the cells were collected by centrifugation, washed 1x with sterile PBS, back diluted to ~3x105CFU/ ml into fresh broth, and immediately incubated at 37oC in a 5% CO2 chamber for 45 minutes. At this point, the first sample was collected. Subsequently, growth was monitored for approximate

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Elizabeth Joyce 

PROVIDER: E-SMDB-2173 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress