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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Streptomyces fradiae wild type time series (ATCC19609) and overproducing strain KOS155-3C(RUS)

ABSTRACT: RNA timecourse data for Streptomyces fradiae wildtype (ATCC19609) and overproducing strain KOS155-3C(RUS). Strains were grown at 30 C in shake flask cultures in R5 medium with no glucose. RNA samples were harvested over 5 days as tylosin was produced. 12 h RNA samples of each strain were used as the reference sample(green) within their respective timecourses. The WT gDNA vs. 12 h RNA control displays relative gene expression at the beginning of the timecourse. The 12 h RNA control compares initial RNA levels between the WT and overproducer.

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Tina Boussard

PROVIDER: E-SMDB-2231 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reverse engineering of industrial pharmaceutical-producing actinomycete strains using DNA microarrays.

Lum Amy M AM Huang Jianqiang J Hutchinson C Richard CR Kao Camilla M CM

Metabolic engineering 20040701 3

Transcript levels in production cultures of wildtype and classically improved strains of the actinomycete bacteria Saccharopolyspora erythraea and Streptomyces fradiae were monitored using microarrays of the sequenced actinomycete S. coelicolor. Sac. erythraea and S. fradiae synthesize the polyketide antibiotics erythromycin and tylosin, respectively, and the classically improved strains contain unknown overproduction mutations. The Sac. erythraea overproducer was found to express the entire 56- ...[more]

PMID: 15256208

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