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Transcription profiling of Vibrio cholerae N16961 grown in M9 + 0.5 % lactate to OD 0.2, culture was split and one part added 0.6 mM of indicated chitin oligosaccharide

ABSTRACT: Strain N16961 grown in M9 + 0.5 % lactate to OD 0.2. Culture was split and one part added 0.6 mM of indicated chitin oligosaccharide. Samples was isolated after 30 and 60 min. cDNA was prepared from 2 ug RNA and labeled with Cy3 (no induction) and Cy5 (induction with chitin oligosaccharide). Samples from at least two independent experiments and a total of four hybridizations for each chitin oligosaccharide and time.

ORGANISM(S): Vibrio cholerae

SUBMITTER: Karin Meibom

PROVIDER: E-SMDB-2237 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Vibrio cholerae chitin utilization program.

Meibom Karin L KL Li Xibing B XB Nielsen Alex T AT Wu Cheng-Yen CY Roseman Saul S Schoolnik Gary K GK

Proceedings of the National Academy of Sciences of the United States of America 20040201 8

Chitin, an insoluble polymer of GlcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and mutational studies of Vibrio cholerae growing on a natural chitin surface, or with the soluble chitin oligosaccharides (GlcNAc)(2-6), GlcNAc, or the glucosamine dimer (GlcN)2 identified three sets of differentially regulated genes. We show that (i) ChiS, a sensor histidine kinase, regulates expression of the (GlcNAc)(2-6) gene set, including ...[more]

PMID: 14983042

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Chitin oligosaccharide induction

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