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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Vibrio cholerae strain N16961 was incubated with crab shell in artificial sea water media for 24 hours

ABSTRACT: Strain N16961 was incubated with crab shell in artificial seawater media for 24 hours. cDNA from 1 ug RNA was labeled with Cy3 (planktonic bacteria) and Cy5 (crab attached bacteria).

ORGANISM(S): Vibrio cholerae

SUBMITTER: Karin Meibom

PROVIDER: E-SMDB-2238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Vibrio cholerae chitin utilization program.

Meibom Karin L KL Li Xibing B XB Nielsen Alex T AT Wu Cheng-Yen CY Roseman Saul S Schoolnik Gary K GK

Proceedings of the National Academy of Sciences of the United States of America 20040201 8

Chitin, an insoluble polymer of GlcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and mutational studies of Vibrio cholerae growing on a natural chitin surface, or with the soluble chitin oligosaccharides (GlcNAc)(2-6), GlcNAc, or the glucosamine dimer (GlcN)2 identified three sets of differentially regulated genes. We show that (i) ChiS, a sensor histidine kinase, regulates expression of the (GlcNAc)(2-6) gene set, including ...[more]

PMID: 14983042

Similar Datasets

Crab shell attachment

Project description:Strain N16961 was incubated with crab shell in artificial seawater media for 24 hours. cDNA from 1 ug RNA was labeled with Cy3 (planktonic bacteria) and Cy5 (crab attached bacteria). A growth condition experiment design type is where some part of the growth condition is changed for the purposes of the experiment, examples of growth conditions changed are media, temperature, humidity, light, nutrients. Keywords: growth_condition_design

2005-08-11 | GSE3102 | GEO

Crab shell attachment

2005-08-11 | E-GEOD-3102 | biostudies-arrayexpress

Gene expression profile of Vibrio cholerae N16961 grown in various conditions of culture

Project description:To investigate the effect of conditions of culture on gene expression in Vibrio cholerae N16961 We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 conditions of culture in biological duplicate

2023-02-20 | GSE214813 | GEO

Comparative genomic hybridization of Vibrio cholerae which have converted from serogroup 01 to 0139 (phage selected) - part 2

Project description:These experiments were performed to show a serogroup conversion of Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 El Tor (A1552) was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (4 ug each) from strain MO10, an O139 serogroup strain, was added after 24h and the cells were further grown for 24h. After detachment from the crab shell fragments, bacteria were poured into soft-agar and overlaid onto LB plates. Mukerjees El Tor phage V (a gift of Dr. M.S. Islam) was dropped onto the surface of the bacteria containing soft-agar. The plaques formed by killing non-transformed A1552 cells possessed resistant clones which were picked and further selected for opaque morphotype and agglutination by O139-specific antiserum. Four clones were selected from each independent experiment and analyzed by microarray hybridization (BioPrime. Array CGH Genomic Labeling from Invitrogen). Two microarray replicates were done per clone. Strain Names: AIIIpO139#1 / AIIIpO139#3 / AIIIpO139#4 / AIIIpO139#5 are four clones analyzed after the second experiment; AIVpO139#2 / AIVpO139#4 / AIVpO139#5 / AIVpO139#8 are four clones analyzed after the fourth independent experiment. Two MA replicates for each clone were done.

2007-07-25 | E-SMDB-3821 | biostudies-arrayexpress

Comparative genomic hybridization of Vibrio cholerae which have converted from serogroup 01 to 0139 (phage selected)

Project description:These experiments were performed to show a serogroup conversion of Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 El Tor (A1552) was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (4 ug each) from strain MO10, an O139 serogroup strain, was added after 24h and the cells were further grown for 24h. After detachment from the crab shell fragments, bacteria were poured into soft-agar and overlaid onto LB plates. Mukerjeee's El Tor phage V (a gift of Dr. M.S. Islam) was dropped onto the surface of the bacteria containing soft-agar. The plaques formed by killing non-transformed A1552 cells possessed resistant clones which were picked and further selected for opaque morphotype and agglutination by O139-specific antiserum. Four clones were selected from each independent experiment and analyzed by microarray hybridization (BioPrime. Array CGH Genomic Labeling from Invitrogen). Two microarray replicates were done per clone. Strain Names: ApO139#2 / ApO139#4 / ApO139#6 / ApO139#8 are four clones analyzed after the first experiment; AIIpO139#3 / AIIpO139#4 / AIIpO139#5 / AIIpO139#6 are four clones analyzed after the second independent experiment. Two MA replicates for each clone were done. CGHs of A1552 versus MO10 are provided as control.

2007-08-01 | E-SMDB-3752 | biostudies-arrayexpress

Vibrio cholerae

Project description:Crab shell attachment

| PRJNA104787 | ENA

Comparative genomic hybridization of Vibrio cholerae from California coastal water compared to reference strain N16961

Project description:Environmental isolates of Vibrio cholerae from California coastal water compared to reference strain N16961.

2007-05-23 | E-SMDB-4063 | biostudies-arrayexpress

crab metagenome

Project description:Crab associated bacteria Metagenome

| PRJNA296845 | ENA

Vibrio cholerae O1 biovar El Tor str. N16961

Project description:Vibrio cholerae N16961 overexpression of CpxR

| PRJNA234543 | ENA

Vibrio cholerae Expression Study

Project description:Question Addressed: What is the level of expression of genes in Vibrio cholerae recovered from various conditions. These conditions include samples recovered directly from patients (O139 from stool samples from ICDDR,B and N16961 from stool samples from a vaccine trial held in Cincinnati) as well as standard logarithmic and stationary phase grown bacteria. Labeling reactions were performed in duplicate for each stool derived and in quadruplicate for each in vitro grown strain. A common reference was used for each slide, it was composed of RNA from the exponentially growing 92A1552 V. cholerae strain

2013-05-23 | GSE38178 | GEO

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