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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Saccharopolyspora erythraea NRRL2338 (wild type) and K41-135 (overproducer strain from Kosan Biosciences, KOVP) grown in shake flask cultures in rich medium R5, time series

ABSTRACT: Saccharopolyspora erythraea NRRL2338 (wildtype) and K41-135 (overproducer strain from Kosan Biosciences, KOVP) were grown in shake flask cultures in rich medium R5. RNA samples were harvested for each strain over 5 days. 12 h samples taken for each strain were used as their respective reference ("time zero") samples. The genomic DNA control compares NRRL2338 gDNA to NRRL2338 12 h RNA to show relative gene expression at the start of the timecourse. The 12 h RNA control compares initial gene expression between the wildtype and overproducer strains.

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Tina Boussard

PROVIDER: E-SMDB-2243 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reverse engineering of industrial pharmaceutical-producing actinomycete strains using DNA microarrays.

Lum Amy M AM Huang Jianqiang J Hutchinson C Richard CR Kao Camilla M CM

Metabolic engineering 20040701 3

Transcript levels in production cultures of wildtype and classically improved strains of the actinomycete bacteria Saccharopolyspora erythraea and Streptomyces fradiae were monitored using microarrays of the sequenced actinomycete S. coelicolor. Sac. erythraea and S. fradiae synthesize the polyketide antibiotics erythromycin and tylosin, respectively, and the classically improved strains contain unknown overproduction mutations. The Sac. erythraea overproducer was found to express the entire 56- ...[more]

PMID: 15256208

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