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Transcription profiling of S. cervisiae wild type (BY4741) and Puf3 mutant cells grown in minimal medium supplemented with 3% glycerol

ABSTRACT: Three independent experiments: S. cervisiae wild-type (BY4741) and Puf3 mutant cells were grown in minimal medium supplemented with 3% glycerol. Cells were harvested in mid-log phase by centrifugation and total RNA was prepared by hot-phenol extraction. cDNA was prepared with oligo-dT and using a mixture of amino-allyl dUTP and dNTPs, fluorescently labeled (Cy3= wild-type, Cy5 = mutant) and hybridize on S. cerevisiae DNA microarray.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-2623 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast.

Gerber André P AP   Herschlag Daniel D   Brown Patrick O PO  

PLoS biology 20040316 3

Genes encoding RNA-binding proteins are diverse and abundant in eukaryotic genomes. Although some have been shown to have roles in post-transcriptional regulation of the expression of specific genes, few of these proteins have been studied systematically. We have used an affinity tag to isolate each of the five members of the Puf family of RNA-binding proteins in Saccharomyces cerevisiae and DNA microarrays to comprehensively identify the associated mRNAs. Distinct groups of 40-220 different mRN  ...[more]

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