Unknown,Transcriptomics,Genomics,Proteomics

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Comparative genomic hybridization of amplified whole tumour genomes from limited sources of fresh frozen and paraffin-embedded DNA (Bredel 2)

ABSTRACT: created to publish for Bredel et al 2005

ORGANISM(S): Homo sapiens

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3334 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Amplification of whole tumor genomes and gene-by-gene mapping of genomic aberrations from limited sources of fresh-frozen and paraffin-embedded DNA.

Bredel Markus M   Bredel Claudia C   Juric Dejan D   Kim Young Y   Vogel Hannes H   Harsh Griffith R GR   Recht Lawrence D LD   Pollack Jonathan R JR   Sikic Branimir I BI  

The Journal of molecular diagnostics : JMD 20050501 2

Sufficient quantity of genomic DNA can be a bottleneck in genome-wide analysis of clinical tissue samples. DNA polymerase Phi29 can be used for the random-primed amplification of whole genomes, although the amplification may introduce bias in gene dosage. We have performed a detailed investigation of this technique in archival fresh-frozen and formalin-fixed/paraffin-embedded tumor DNA by using cDNA microarray-based comparative genomic hybridization. Phi29 amplified DNA from matched pairs of fre  ...[more]

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