Transcription profiling of famililal Mediterranean fever (FMF) to investigate the effect of colchicine

ABSTRACT: Objective. Colchicine is an alkaloid that is used to alleviate acute gout and to prevent acute attacks of familial Mediterranean fever (FMF). However, it is not beneficial when given during the occurrence of an acute episode of FMF. It is believed that colchicine exerts its anti-inflammatory effect through direct interaction with microtubules. We aim to study the molecular basis of colchicine action by analysing the effect of this drug on global gene expression of HUVEC (human umbilical vein endothelial cell line) cells. Methods. HUVEC cells were exposed to various concentrations of colchicine and were harvested at different time points. Ribonucleic acid was extracted, amplified, reverse transcribed and hybridized to complementary deoxyribonucleic acid microarrrays containing more than 40,

ORGANISM(S): Homo sapiens

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress