Transcription profiling of mechanical shear effects on flexor tendon cells

ABSTRACT: Adhesion formation after flexor tendon repair remains a clinical problem. Early postoperative motion after tendon repair has been demonstrated to reduce adhesion formation while increasing tendon strength. It is hypothesized that during mobilization, tendon cells experience mechanical shear forces that alter their biology in a fashion that reduces scar formation but also activates key genes involved in tendon healing. To test this hypothesis, primary intrinsic tenocyte cultures were established from flexor tendons of 20 Sprague-Dawley rats and sheared at 50 rpm (0.41 Pa) using a cone viscometer for 6 and 12 hours. Total RNA was harvested and compared with time-matched unsheared controls using cDNA microarrays and Northern blot analysis. Microarray analysis demonstrated that mechanical shea

ORGANISM(S): Mus musculus

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3543 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress