Comparative genomic hybridization of Vibrio cholerae serogroup conversion from 01 to 0139

ABSTRACT: These experiment were performed to show a serogroup conversion in Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 WT = A1552 was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (2 ug each) from strain VCO139-Kan was added after 24h and the cells grwon further for 24h. The VCO139-Kan strain is a MO10 derivative (both O139 serogroup) which harbors a Kanamycin cassette in the O139 region (as part of the operon between wbfA and wbfB w/o own promotor) for better selection. Transformants were selected on LB+Kan plates. Two groups of transformants were gained: Group I had a full exchange of the O1 region by the O139 region (clones serogroup-converted: SGC#1-3); the crossovers for the homologous recombination event had occurred withi

ORGANISM(S): Vibrio cholerae

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3574 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress