Transcription profiling of Drosophila melanogaster Upf225G/Y Btl-GAL4, UAS-GFP/+ and y w FRT19A/ Y Btl-GAL4, UAS-GFP/+ L3 larvae, Upf225G mutants are delayed in development

ABSTRACT: RNA was isolated from Upf225G/Y; Btl-GAL4, UAS-GFP/+ and y w FRT19A/ Y; Btl-GAL4, UAS-GFP/+ L3 larvae using Trizol as described in Materials and Methods, cDNA labeled with Cy3 or Cy5 was prepared from each RNA sample and hybridized to microarrays containing ~14,000 gene probes covering the entire Drosophila genome (Gerber et al. 2006). Hybridizations were performed with two independently isolated and labeled RNA samples. Analysis was carried out using the Stanford Microarray Database (http://genome-www5.stanford.edu/ ). During analysis we noted that the Upf225G mutants were delayed in development. To avoid confounding effects of changes in gene expression that result from developmental regulation rather than more direct effects of Upf2 loss of function, we used the available wild- type

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress