Transcription profiling of human embryonic stem cells during differentiation to more restricted neural progenitors

ABSTRACT: The purpose of this study was to examine global gene expression during the differentiation of human embryonic stem cells to more restricted neural progenitors. We developed an adherent differentiation protocol with completely defined media that produced 2 distinct classes of neuroepithelia based on timing, morphology and expression of known neural markers. The first stage of differentiation is undifferentiated human ES cells (hESCs). The second stage is aggregates of these hESCs after 6 days of separation from supportive mouse embryonic fibroblasts. The third stage is a primitive anterior neuroepithelia that arises after 10 days of differentiation and is marked by a columnar morphology, expression of Pax6 and anterior neural patterning genes. The fourth stage peaks at 17 days when cel

ORGANISM(S): Homo sapiens

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress