Comparative genomic hybridization of kanmycin selected Vibrio cholerae with serogroup 01 and those converted from serogroup 01 to 037

ABSTRACT: These experiments were performed to show a serogroup conversion in Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 WT = A1552 was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (2 ug each) from strain VC73-orf6/7-Kan-A was added after 24h and the cells grown further for 24h. The VC73-orf6/7-Kan-A strain is a ATCC25873 derivative (both O37 serogroup) which harbors a Kanamycin cassette in the O37 region (as part of the operon between orf6 and orf7 w/o own promotor) for better selection. Transformants were selected on LB+Kan plates. Three clones were selected from each experiment and analyzed by microarray hybridization (BioPrime. Array CGH Genomic Labeling from Invitrogen). Two microarray replicates were done per clone. Compari

ORGANISM(S): Vibrio cholerae

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3849 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress