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Transcription profiling of basal epidermis derived from UVB-irradiated P2.5 normal, Mc1r-mutant and Kit-mutant mice

ABSTRACT: Total RNA was extracted from the basal epidermis derived from UVB-irradiated P2.5 normal (Mc1r+/Mc1r+; Kit+/Kit+), Mc1r-mutant (Mc1re/Mc1re) and Kit-mutant (Kit/W-v/Kit/W-v) mice. Biological replicates (cy5) were labelled and co-hybridized with a reference RNA sample (cy3) to a 35K mouse cDNA microarray.

ORGANISM(S): Mus musculus

SUBMITTER: Janos Demeter

PROVIDER: E-SMDB-3976 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Distinct pigmentary and melanocortin 1 receptor-dependent components of cutaneous defense against ultraviolet radiation.

April Craig S CS Barsh Gregory S GS

PLoS genetics 20061201 1

Genetic variation at the melanocortin 1 receptor (MC1R) is an important risk factor for developing ultraviolet (UV) radiation-induced skin cancer, the most common form of cancer in humans. The underlying mechanisms by which the MC1R defends against UV-induced skin cancer are not known. We used neonatal mouse skin (which, like human skin, contains a mixture of melanocytes and keratinocytes) to study how pigment cells and Mc1r genotype affect the genome-level response to UV radiation. Animals with ...[more]

PMID: 17222061

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